Issue 44, 2012
From the journal:

Chemical Communications

Stepping towards highly flexible aptamers: enzymatic recognition studies of unlocked nucleic acid nucleotides†

Camille Dubois,a   Meghan A. Campbell,b   Stacey L. Edwards,a   Jesper Wengelb  and  Rakesh N. Veedu*a  

* Corresponding authors

a School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, Brisbane, Australia
E-mail: rakesh@uq.edu.au
Fax: +61 7 33654699
Tel: +61 7 33654611

b Nucleic Acid Center, Department of Physics and Chemistry, University of Southern Denmark, Campusvej 55, Odense M, Denmark

Abstract

Enzymatic recognition of unlocked nucleic acid (UNA) nucleotides was successfully accomplished. Therminator DNA polymerase was found to be an efficient enzyme in primer extension reactions. Polymerase chain reaction (PCR) amplification of a 81 mer UNA-modified DNA library was efficiently achieved by KOD DNA polymerase.

Graphical abstract: Stepping towards highly flexible aptamers: enzymatic recognition studies of unlocked nucleic acid nucleotides

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Article information

DOI
https://doi.org/10.1039/C2CC31316B
Article type
Communication
Submitted
22 Feb 2012
Accepted
03 Apr 2012
First published
05 Apr 2012

Chem. Commun., 2012,48, 5503-5505

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Stepping towards highly flexible aptamers: enzymatic recognition studies of unlocked nucleic acid nucleotides

C. Dubois, M. A. Campbell, S. L. Edwards, J. Wengel and R. N. Veedu, Chem. Commun., 2012, 48, 5503 DOI: 10.1039/C2CC31316B

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