Issue 45, 2012
From the journal:

Chemical Communications

Hybridization-triggered isothermal signal amplification coupled with MutS for label-free and sensitive fluorescent assay of SNPs†

Anli Jiao,‡a   Jing Zheng,‡ab   Yaping Hu,a   Guizhi Zhu,b   Jishan Li,*a   Huimin Li,*a   Ronghua Yang*a  and  Weihong Tanab  

* Corresponding authors

a State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, and College of Materials and Engineering, Hunan University, Changsha, P. R. China
E-mail: jishanli@hnu.cn, hnhuimin@yahoo.com, Yangrh@pku.edu.cn
Fax: (86)731-88822523
Tel: (86)731-88822523

b Center for Research at the Bio/Nano Interface, Department of Chemistry and Department of Physiology and Functional Genomics, Shands Cancer Center and UF Genetics Institute, University of Florida, Gainesville, Florida, USA

Abstract

Combining the specific recognization of MutS protein for mismatched DNA sequences with the target-driven molecular switch that acts as both the template and primer of the polymerization reaction, a new label-free and sensitive fluorescent assay strategy for specific single-stranded DNA sequences or SNPs is proposed.

Graphical abstract: Hybridization-triggered isothermal signal amplification coupled with MutS for label-free and sensitive fluorescent assay of SNPs

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Supplementary files

Article information

DOI
https://doi.org/10.1039/C2CC30882G
Article type
Communication
Submitted
08 Feb 2012
Accepted
16 Apr 2012
First published
17 Apr 2012

Chem. Commun., 2012,48, 5659-5661

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Hybridization-triggered isothermal signal amplification coupled with MutS for label-free and sensitive fluorescent assay of SNPs

A. Jiao, J. Zheng, Y. Hu, G. Zhu, J. Li, H. Li, R. Yang and W. Tan, Chem. Commun., 2012, 48, 5659 DOI: 10.1039/C2CC30882G

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