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Issue 10, 2012
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An optimized isotopic labelling strategy of isoleucine-γ2 methyl groups for solution NMR studies of high molecular weight proteins

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Abstract

An efficient synthetic route is proposed to produce 2-hydroxy-2-ethyl-3-oxobutanoate for the specific labelling of Ile methyl-γ2groups in proteins. The 2H, 13C-pattern of the biosynthetic precursor has been designed to optimize magnetization transfer, in large proteins, between these important structural probes and their corresponding backbone nuclei.

Graphical abstract: An optimized isotopic labelling strategy of isoleucine-γ2 methyl groups for solution NMR studies of high molecular weight proteins

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Supplementary files

Article information


Submitted
18 May 2011
Accepted
14 Jul 2011
First published
26 Jul 2011

Chem. Commun., 2012,48, 1434-1436
Article type
Communication

An optimized isotopic labelling strategy of isoleucine-γ2 methyl groups for solution NMR studies of high molecular weight proteins

I. Ayala, O. Hamelin, C. Amero, O. Pessey, M. J. Plevin, P. Gans and J. Boisbouvier, Chem. Commun., 2012, 48, 1434 DOI: 10.1039/C1CC12932E

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