Issue 43, 2011
From the journal:

Chemical Communications

Light-initiated hydroxylation of lauric acid using hybrid P450 BM3 enzymes†

Ngoc-Han Tran,a   Ngoc Huynh,a   Thuba Bui,a   Yen Nguyen,a   Phuong Huynh,a   Mary E. Coopera  and  Lionel E. Cheruzel*a  

* Corresponding authors

a San José State University, Department of Chemistry, One Washington Square, San José, USA
E-mail: lionel.cheruzel@sjsu.edu
Fax: +1 408 924 4945
Tel: +1 408 924 5283

Abstract

We have developed hybrid P450 BM3 enzymes consisting of a Ru(II)-diimine photosensitizer covalently attached to non-native single cysteine residues of P450 BM3 heme domain mutants. These enzymes are capable, upon light activation, of selectively hydroxylating lauric acid with 40 times higher total turnover numbers compared to the peroxide shunt.

Graphical abstract: Light-initiated hydroxylation of lauric acid using hybrid P450 BM3 enzymes

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Article information

DOI
https://doi.org/10.1039/C1CC15124J
Article type
Communication
Submitted
17 Aug 2011
Accepted
15 Sep 2011
First published
05 Oct 2011

Chem. Commun., 2011,47, 11936-11938

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Light-initiated hydroxylation of lauric acid using hybrid P450 BM3 enzymes

N. Tran, N. Huynh, T. Bui, Y. Nguyen, P. Huynh, M. E. Cooper and L. E. Cheruzel, Chem. Commun., 2011, 47, 11936 DOI: 10.1039/C1CC15124J

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