A water soluble vitamin B12-Re(I) fluorescent conjugate for cell uptake screens: use in the confirmation of cubilin in the lung cancer line A549

Anthony R. Vortherms; Anna R. Kahkoska; Amy E. Rabideau; Jon Zubieta; Louise Lund Andersen; Mette Madsen; Robert P. Doyle

doi:10.1039/C1CC13615A

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A water soluble vitamin B₁₂-Re(i) fluorescent conjugate for cell uptake screens: use in the confirmation of cubilin in the lung cancer line A549†

Anthony R. Vortherms,‡^a Anna R. Kahkoska,^a Amy E. Rabideau,§^a Jon Zubieta,^a Louise Lund Andersen,^b Mette Madsen^b and Robert P. Doyle*^a

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* Corresponding authors

^a Department of Chemistry, Centre for Science and Technology, Syracuse University, Syracuse, NY 13244, USA
E-mail: rpdoyle@syr.edu
Fax: +315 443 4070
Tel: +315 443 3584

^b Department of Medical Biochemistry, University of Aarhus, Ole Worms Allé, Bldg. 1170-1171, DK-8000 Aarhus C, Denmark

Abstract

A water soluble vitamin B₁₂-rhenium conjugate was synthesized and used in concert with intrinsic factor to screen for cubilin receptor-mediated uptake in lung cancer cells. Internalization of the conjugate demonstrated that it could be used to rapidly screen for the cubilin receptor in living cells, subsequently confirmed with Western blotting and RT-PCR.

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Article information

DOI: https://doi.org/10.1039/C1CC13615A
Article type: Communication
Submitted: 17 Jun 2011
Accepted: 21 Jul 2011
First published: 05 Aug 2011

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Chem. Commun., 2011,47, 9792-9794

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A water soluble vitamin B₁₂-Re(I) fluorescent conjugate for cell uptake screens: use in the confirmation of cubilin in the lung cancer line A549

A. R. Vortherms, A. R. Kahkoska, A. E. Rabideau, J. Zubieta, L. L. Andersen, M. Madsen and R. P. Doyle, Chem. Commun., 2011, 47, 9792 DOI: 10.1039/C1CC13615A

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