Issue 30, 2011

Highly efficient enzymatic synthesis of 3′-deoxyapionucleic acid (apioNA) having the four natural nucleobases

Abstract

The synthesis of the 3′-deoxyapionucleoside 3′′-triphosphates (apioNTPs) having the four natural nucleobases and their enzymatic incorporation into a DNA–DNA primer–template have been tried. Therminator DNA polymerase was shown to incorporate these apioNTPs effectively giving 43mer DNA–apioNA chimera.

Graphical abstract: Highly efficient enzymatic synthesis of 3′-deoxyapionucleic acid (apioNA) having the four natural nucleobases

Supplementary files

Article information

Article type
Communication
Submitted
21 May 2011
Accepted
21 Jun 2011
First published
04 Jul 2011

Chem. Commun., 2011,47, 8700-8702

Highly efficient enzymatic synthesis of 3′-deoxyapionucleic acid (apioNA) having the four natural nucleobases

M. Kataoka, Y. Kouda, K. Sato, N. Minakawa and A. Matsuda, Chem. Commun., 2011, 47, 8700 DOI: 10.1039/C1CC12980E

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