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Issue 20, 2011
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Structure-guided redesign of D-fructose-6-phosphate aldolase from E. coli: remarkable activity and selectivity towards acceptor substrates by two-point mutation

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Abstract

Structure-guided re-design of the acceptor binding site of D-fructose-6-phosphate aldolase from E. coli leads to the construction of FSA A129S/A165G double mutant with an activity between 5- to >900-fold higher than that of wild-type towards N-Cbz-aminoaldehyde derivatives.

Graphical abstract: Structure-guided redesign of d-fructose-6-phosphate aldolase from E. coli: remarkable activity and selectivity towards acceptor substrates by two-point mutation

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Publication details

The article was received on 23 Feb 2011, accepted on 31 Mar 2011 and first published on 18 Apr 2011


Article type: Communication
DOI: 10.1039/C1CC11069A
Chem. Commun., 2011,47, 5762-5764

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    Structure-guided redesign of D-fructose-6-phosphate aldolase from E. coli: remarkable activity and selectivity towards acceptor substrates by two-point mutation

    M. Gutierrez, T. Parella, J. Joglar, J. Bujons and P. Clapés, Chem. Commun., 2011, 47, 5762
    DOI: 10.1039/C1CC11069A

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