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Issue 11, 2011
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Modification of transmembrane and GPI-anchored proteins on living cells by efficient proteintrans-splicing using the Npu DnaE intein

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Abstract

The naturally split Npu DnaE intein can be used for ligation of an exogenous polypeptide to membrane proteins on living cells. No reducing agents or other factors are required. The approach is rapid and virtually traceless, because the intein removes itself during the reaction.

Graphical abstract: Modification of transmembrane and GPI-anchored proteins on living cells by efficient proteintrans-splicing using the Npu DnaE intein

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Supplementary files

Article information


Submitted
01 Oct 2010
Accepted
22 Dec 2010
First published
13 Jan 2011

Chem. Commun., 2011,47, 3063-3065
Article type
Communication

Modification of transmembrane and GPI-anchored proteins on living cells by efficient proteintrans-splicing using the Npu DnaE intein

T. Dhar and H. D. Mootz, Chem. Commun., 2011, 47, 3063
DOI: 10.1039/C0CC04172F

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