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Issue 6, 2011
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Incorporation of chlorinated analogues of aliphatic amino acids during cell-free protein synthesis

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Abstract

3-Chloro-Abu and 4-chloro-Nva are biosynthetically incorporated into E. coli peptidyl-Pro cis-trans isomerase B, as substitutes for Val and Leu, respectively. The extent of incorporation is up to ∼90%, and substituted protein is catalytically active. By contrast, 4-chloro-Val is not an effective replacement for Ile.

Graphical abstract: Incorporation of chlorinated analogues of aliphatic amino acids during cell-free protein synthesis

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Article information


Submitted
29 Jul 2010
Accepted
30 Oct 2010
First published
22 Nov 2010

Chem. Commun., 2011,47, 1839-1841
Article type
Communication

Incorporation of chlorinated analogues of aliphatic amino acids during cell-free protein synthesis

D. J. Stigers, Z. I. Watts, J. E. Hennessy, H. Kim, R. Martini, M. C. Taylor, K. Ozawa, J. W. Keillor, N. E. Dixon and C. J. Easton, Chem. Commun., 2011, 47, 1839
DOI: 10.1039/C0CC02879G

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