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Issue 6, 2011
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Incorporation of chlorinated analogues of aliphatic amino acids during cell-free protein synthesis

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Abstract

3-Chloro-Abu and 4-chloro-Nva are biosynthetically incorporated into E. coli peptidyl-Pro cis-trans isomerase B, as substitutes for Val and Leu, respectively. The extent of incorporation is up to ∼90%, and substituted protein is catalytically active. By contrast, 4-chloro-Val is not an effective replacement for Ile.

Graphical abstract: Incorporation of chlorinated analogues of aliphatic amino acids during cell-free protein synthesis

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Publication details

The article was received on 29 Jul 2010, accepted on 30 Oct 2010 and first published on 22 Nov 2010


Article type: Communication
DOI: 10.1039/C0CC02879G
Chem. Commun., 2011,47, 1839-1841

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    Incorporation of chlorinated analogues of aliphatic amino acids during cell-free protein synthesis

    D. J. Stigers, Z. I. Watts, J. E. Hennessy, H. Kim, R. Martini, M. C. Taylor, K. Ozawa, J. W. Keillor, N. E. Dixon and C. J. Easton, Chem. Commun., 2011, 47, 1839
    DOI: 10.1039/C0CC02879G

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