Issue 4, 2011
From the journal:

Chemical Communications

Aptamer-based turn-on fluorescent four-branched quaternary ammonium pyrazine probe for selective thrombin detection†

Shengyong Yan,‡a   Rong Huang,‡a   Yangyang Zhou,a   Ming Zhang,a   Minggang Deng,a   Xiaolin Wang,a   Xiaocheng Wenga  and  Xiang Zhou*ab  

* Corresponding authors

a College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, State Key Laboratory of Virology, Wuhan University, Hubei, Wuhan, P. R. China
E-mail: xzhou@whu.edu.cn
Fax: +86 27-87336380
Tel: +86 27-61056559

b State Key Laboratory of Natural and Biomimetic Drugs, Peking University, China

Abstract

In this thrombin detection system, the bright fluorescence of TASPI is almost eliminated by the DNA aptamer TBA (turn-off); however, in the presence of thrombin, it specifically binds to TBA by folding unrestricted TBA into an anti-parallel G-quadruplex structure and then releasing TASPI molecules, resulting in vivid and facile fluorescence recovery (turn-on).

Graphical abstract: Aptamer-based turn-on fluorescent four-branched quaternary ammonium pyrazine probe for selective thrombin detection

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Article information

DOI
https://doi.org/10.1039/C0CC02792H
Article type
Communication
Submitted
25 Jul 2010
Accepted
03 Nov 2010
First published
22 Nov 2010

Chem. Commun., 2011,47, 1273-1275

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Aptamer-based turn-on fluorescent four-branched quaternary ammonium pyrazine probe for selective thrombin detection

S. Yan, R. Huang, Y. Zhou, M. Zhang, M. Deng, X. Wang, X. Weng and X. Zhou, Chem. Commun., 2011, 47, 1273 DOI: 10.1039/C0CC02792H

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