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Issue 39, 2010
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Diazirine-containing RNA photocrosslinking probes for the study of siRNAprotein interactions

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Abstract

We here report the synthesis and characterization of small interfering RNAs with aryl trifluoromethyl diazirine moieties in the 3′-overhang regions, which allow sensitive detection of interacting proteins during assembly of the effector ribonucleoprotein complex by irradiation with minimally destructive long-wavelength ultraviolet light.

Graphical abstract: Diazirine-containing RNA photocrosslinking probes for the study of siRNA–protein interactions

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Publication details

The article was received on 09 Jul 2010, accepted on 16 Aug 2010 and first published on 06 Sep 2010


Article type: Communication
DOI: 10.1039/C0CC02450C
Chem. Commun., 2010,46, 7367-7369

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    Diazirine-containing RNA photocrosslinking probes for the study of siRNAprotein interactions

    S. Kuboe, M. Yoda, A. Ogata, Y. Kitade, Y. Tomari and Y. Ueno, Chem. Commun., 2010, 46, 7367
    DOI: 10.1039/C0CC02450C

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