Issue 39, 2010

Turn-on fluorescence switch involving aggregation and elimination processes for β-lactamase-tag

Abstract

The targeted protein of interest is fused with genetically modified β-lactamase enzyme, which reacts with the probe in physiological conditions to break the aggregated interaction between the fluorophore and quencher. This alliance–separation technique is new for protein labeling and is probed in vitro and in live cell imaging studies.

Graphical abstract: Turn-on fluorescence switch involving aggregation and elimination processes for β-lactamase-tag

Supplementary files

Article information

Article type
Communication
Submitted
08 Jul 2010
Accepted
13 Aug 2010
First published
08 Sep 2010

Chem. Commun., 2010,46, 7403-7405

Turn-on fluorescence switch involving aggregation and elimination processes for β-lactamase-tag

K. K. Sadhu, S. Mizukami, S. Watanabe and K. Kikuchi, Chem. Commun., 2010, 46, 7403 DOI: 10.1039/C0CC02432E

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