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Issue 27, 2009
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Highly sensitive mutation detection based on digital amplification coupled with hydrogel bead-array

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Abstract

In order to detect small amounts of mutants for early cancer diagnosis, we have developed the novel method of using amplicon-coated microbeads and single-molecule-PCR in water-in-oil emulsions, which we coupled with a new detection platform, the hydrogel bead-array, a 3-D polyacrylamidegel network used as a carrier to immobilize the beads.

Graphical abstract: Highly sensitive mutation detection based on digital amplification coupled with hydrogel bead-array

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Article information


Submitted
02 Feb 2009
Accepted
13 May 2009
First published
10 Jun 2009

Chem. Commun., 2009, 4094-4096
Article type
Communication

Highly sensitive mutation detection based on digital amplification coupled with hydrogel bead-array

H. Huang, Z. Qi, L. Deng, G. Zhou, T. Kajiyama and H. Kambara, Chem. Commun., 2009, 4094
DOI: 10.1039/B902196E

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