Issue 21, 2007
From the journal:

Chemical Communications

Dual-labeled oligonucleotide probe for sensing adenosinevia FRET: A novel alternative to SNPs genotyping†

Yoshio Saito,*a   Subhendu Sekhar Bag,b   Yuichi Kusakabe,a   Chiharu Nagai,a   Katsuhiko Matsumoto,a   Erika Mizuno,a   Satoshi Kodate,a   Isamu Suzukaa  and  Isao Saito*b  

* Corresponding authors

a Department of Materials Chemistry and Engineering, School of Engineering, Nihon University, Koriyama, Fukushima 963-8642, Japan
E-mail: saitoy@chem.ce.nihon-u.ac.jp

b NEWCAT Institute, Graduate School of Engineering, Nihon University and SORST Japan Science and Technology Agency, Koriyama, Fukushima 963-8642, Japan
E-mail: saito@mech.ce.nihon-u.ac.jp
Fax: +81-24-956-8924
Tel: +81-24-956-8923

Abstract

A novel FRET based strategy for DNA sequence analysis utilising base-discriminating fluorescence (BDF) nucleoside, PyU/2-AntU, as donor in the dual-labelled oligonucleotide probe is reported; a selective/specific emission from acceptor, was observed upon excitation at the donor, only when the opposite base of the “smart” fluorescently labeled BDF nucleoside, PyU/2-AntU, is adenine on the complementary target sequence.

Graphical abstract: Dual-labeled oligonucleotide probe for sensing adenosinevia FRET: A novel alternative to SNPs genotyping

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Article information

DOI
https://doi.org/10.1039/B618465K
Article type
Communication
Submitted
18 Dec 2006
Accepted
01 Feb 2007
First published
28 Feb 2007

Chem. Commun., 2007, 2133-2135

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Dual-labeled oligonucleotide probe for sensing adenosinevia FRET: A novel alternative to SNPs genotyping

Y. Saito, S. S. Bag, Y. Kusakabe, C. Nagai, K. Matsumoto, E. Mizuno, S. Kodate, I. Suzuka and I. Saito, Chem. Commun., 2007, 2133 DOI: 10.1039/B618465K

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