Promotion of stable triplex formation by partial incorporation of 2′,5′-phosphodiester linkages into triplex-forming oligonucleotides

Abstract

Pentadecamer homopyrimidine oligonucleotides containing three or more 2′,5′-phosphodiester linkages in different modes were prepared and used to evaluate the ability as a triplex-forming oligonucleotide (TFO), and it was found that discontinuous replacement of the 3′,5′-phosphodiester linkages in TFO by 2′,5′-linkages significantly stabilizes parallel-motif triplexes.