Issue 24, 2001
From the journal:

Chemical Communications

Complete reversal of enantioselectivity of an enzyme-catalyzed reaction by directed evolution

Dongxing Zha,a   Stephanie Wilensek,a   Markus Hermes,a   Karl-Erich- Jaegerb  and  Manfred T. Reetz*a  

* Corresponding authors

a Max-Planck-Institut für Kohlenforschung, Mülheim/Ruhr, Germany.
E-mail: reetz@mpi-muelheim.mpg.de;
Fax: +49 208 306 2985

b Ruhr-Universität Bochum, Lehrstuhl der Mikroorganismen, Bochum, Germany

Abstract

The combination of error prone PCR at high mutation rate and DNA shuffling can be used to invert the direction of enantioselectivity of a lipase-catalyzed hydrolytic kinetic resolution involving a chiral ester.

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Article information

DOI
https://doi.org/10.1039/B109913M
Article type
Communication
Submitted
30 Oct 2001
Accepted
15 Nov 2001
First published
06 Dec 2001

Chem. Commun., 2001, 2664-2665

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Complete reversal of enantioselectivity of an enzyme-catalyzed reaction by directed evolution

D. Zha, S. Wilensek, M. Hermes, K. Jaeger and M. T. Reetz, Chem. Commun., 2001, 2664 DOI: 10.1039/B109913M

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