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Issue 2, 2020
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Neighborhood watch: tools for defining locale-dependent subproteomes and their contextual signaling activities

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Abstract

Transient associations between numerous organelles—e.g., the endoplasmic reticulum and the mitochondria—forge highly-coordinated, particular environments essential for cross-compartment information flow. Our perspective summarizes chemical–biology tools that have enabled identifying proteins present within these itinerant communities against the bulk proteome, even when a particular protein's presence is fleeting/substoichiometric. However, proteins resident at these ephemeral junctions also experience transitory changes to their interactomes, small-molecule signalomes, and, importantly, functions. Thus, a thorough census of sub-organellar communities necessitates functionally probing context-dependent signaling properties of individual protein-players. Our perspective accordingly further discusses how repurposing of existing tools could allow us to glean a functional understanding of protein-specific signaling activities altered as a result of organelles pulling together. Collectively, our perspective strives to usher new chemical–biology techniques that could, in turn, open doors to modulate functions of specific subproteomes/organellar junctions underlying the nuanced regulatory subsystem broadly termed as contactology.

Graphical abstract: Neighborhood watch: tools for defining locale-dependent subproteomes and their contextual signaling activities

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Article information


Submitted
02 Apr 2020
Accepted
16 May 2020
First published
27 May 2020

This article is Open Access

RSC Chem. Biol., 2020,1, 42-55
Article type
Review Article

Neighborhood watch: tools for defining locale-dependent subproteomes and their contextual signaling activities

M. J. C. Long, Y. Zhao and Y. Aye, RSC Chem. Biol., 2020, 1, 42
DOI: 10.1039/D0CB00041H

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