SCHEDULED MAINTENANCE
Incubation of [(5S)-5-amino-5-carboxypentanoyl]-L-[3-13C]cysteinyl-D-[15N,3-2H]valine with isopenicillin N synthase (IPNS) resulted in the observation of a ‘shunt metabolite’, which we believe is formed from the collapse of an enzyme-bound monocyclic β-lactam intermediate, and which from chemical studies has been shown not to be derived from the decomposition of a free thiol monocyclic β-lactam.
J. E. Baldwin, R. M. Adlington, M. Bradley, W. J. Norris, N. J. Turner and A. Yoshida, J. Chem. Soc., Chem. Commun., 1988, 1125 DOI: 10.1039/C39880001125
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