Jump to main content
Jump to site search

Issue 1, 2020
Previous Article Next Article

3D spheroids generated on carbon nanotube-functionalized fibrous scaffolds for drug metabolism and toxicity screening

Author affiliations

Abstract

The mechanical and electrical stimuli have a profound effect on the cellular behavior and function. In this study, a series of conductive nanofibrous scaffolds are developed by blend electrospinning of poly(styrene-co-maleic acid) (PSMA) and multiwalled-carbon nanotubes (CNTs), followed by grafting galactose as cell adhesion cues. When the mass ratios of CNTs to PSMA increase up to 5%, the alignment, Young's modulus and conductivity of fibrous scaffolds increase, whereas the average diameter, pore size and elongation at break decrease. Primary hepatocytes cultured on the scaffolds are self-assembled into 3D spheroids, which restores the hepatocyte polarity and sufficient expression of drug metabolism enzymes over an extended period of time. Among these conductive scaffolds, hepatocytes cultured on fibers containing 3% of CNTs (F3) show the highest clearance rates of model drugs, offering a better prediction of the in vivo data with a high correlation value. Moreover, the drug metabolism capability is maintained over 15 days and is more sensitive towards the inducers and inhibitors of metabolizing enzymes, demonstrating the applicability for drug–drug interaction studies. Thus, this culture system has been demonstrated as a reliable in vitro model for high-throughput screening of metabolism and toxicity in the early phases of drug development.

Graphical abstract: 3D spheroids generated on carbon nanotube-functionalized fibrous scaffolds for drug metabolism and toxicity screening

Back to tab navigation

Article information


Submitted
19 Aug 2019
Accepted
04 Nov 2019
First published
05 Nov 2019

Biomater. Sci., 2020,8, 426-437
Article type
Paper

3D spheroids generated on carbon nanotube-functionalized fibrous scaffolds for drug metabolism and toxicity screening

J. Wei, J. Lu, M. Chen, S. Xie, T. Wang and X. Li, Biomater. Sci., 2020, 8, 426
DOI: 10.1039/C9BM01310E

Social activity

Search articles by author

Spotlight

Advertisements