Jump to main content
Jump to site search

Issue 6, 2019
Previous Article Next Article

Functional, UV-curable coating for the capture of circulating tumor cells

Author affiliations

Abstract

The capture of circulating tumor cells (CTCs) plays a crucial role in the early diagnosis, personalized treatment and postoperative evaluation of malignant tumors. In this study, UV-curable coating technology was combined with antibody immobilization to enable CTC captures on poly(methyl methacrylate) (PMMA) substrates. Controlled amounts of carboxyl groups and polyethylene glycol (PEG) segments were introduced into the coating formulation to facilitate immobilization of antibodies and block non-specific protein adsorption, respectively. Then, anti-EpCAM antibodies were immobilized on functionalized, coated PMMA substrates by EDC/NHS chemistry. Multiple physical, chemical and biological properties were investigated, including hydrophilicity, protein adsorption, platelet adhesion and anticoagulant properties. Thereafter, optimized coatings were applied on the inner wall of PMMA tubes, followed by immobilization of anti-EpCAM antibodies. After perfusion of the tubes with whole blood, enriched with SGC7901 gastric cancer cells that overexpress EpCAM antigens, rapid and efficient capture of the tumor cells was observed. These results provide a basis for further development of devices for the selective capture and enrichment of CTCs, using small blood volumes.

Graphical abstract: Functional, UV-curable coating for the capture of circulating tumor cells

Back to tab navigation

Article information


Submitted
18 Feb 2019
Accepted
20 Mar 2019
First published
26 Mar 2019

Biomater. Sci., 2019,7, 2383-2393
Article type
Paper

Functional, UV-curable coating for the capture of circulating tumor cells

W. Song, X. Li, Y. Zhao, C. Liu, J. Xu, H. Wang and T. Zhang, Biomater. Sci., 2019, 7, 2383
DOI: 10.1039/C9BM00264B

Social activity

Search articles by author

Spotlight

Advertisements