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Issue 10, 2016
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In vitro detection of human breast cancer cells (SK-BR3) using herceptin-conjugated liquid crystal microdroplets as a sensing platform

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Abstract

The present study utilizes antibody–protein interactions to develop an LC microdroplet based biosensor for naked eye detection of SK-BR3 human breast cancer cells. The herceptin antibody-conjugated LC microdroplets were fabricated using 4-cyano-4′-pentyl biphenyl (5CB) as the liquid crystalline phase and sodium dodecyl sulfate (SDS) as the surfactant. The poly (styrene-b-acrylic acid) amphiphilic block copolymer (PS-b-PA) played a role as a modifier for the liquid crystalline interfaces. The 5CB molecules in the herceptin antibody-conjugated LC microdroplets have shown an orientation transition from radial to bipolar on selective interactions with targeted SK-BR3 breast cancer cells, which are over expressed by the human epidermal growth factor receptor 2-positive (HER2). The herceptin antibody-conjugated LC microdroplets are found to be highly selective in the detection of SK-BR3 cancer cells in the presence of control cells, such as KB cancer cells and fibroblast (FB), and also in the presence of 10% human blood plasma. The interaction forces of the SK-BR3 cancer cells were only effective in causing orientation transitions in 5CB molecules in the LC microdroplets, which clearly suggested that the herceptin antibody-conjugated LC microdroplets could be used as a selective biosensor for a real-time detection of SK-BR3 cancer cells in biological fluids.

Graphical abstract: In vitro detection of human breast cancer cells (SK-BR3) using herceptin-conjugated liquid crystal microdroplets as a sensing platform

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Publication details

The article was received on 15 Jun 2016, accepted on 01 Aug 2016 and first published on 17 Aug 2016


Article type: Paper
DOI: 10.1039/C6BM00404K
Biomater. Sci., 2016,4, 1473-1484

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    In vitro detection of human breast cancer cells (SK-BR3) using herceptin-conjugated liquid crystal microdroplets as a sensing platform

    W. Ding, K. C. Gupta, S. Park, Y. Kim and I. Kang, Biomater. Sci., 2016, 4, 1473
    DOI: 10.1039/C6BM00404K

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