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Issue 3, 2015
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Enhanced cellular uptake of engineered spider silk particles

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Abstract

Drug delivery systems allow tissue/cell specific targeting of drugs in order to reduce total drug amounts administered to an organism and potential side effects upon systemic drug delivery. Most drug delivery systems are polymer-based, but the number of possible materials is limited since many commercially available polymers induce allergic or inflammatory responses or lack either biodegradability or the necessary stability in vivo. Spider silk proteins represent a new class of (bio)polymers that can be used as drug depots or drug delivery systems. The recombinant spider silk protein eADF4(C16), which can be processed into different morphologies such as particles, films, or hydrogels, has been shown to fulfil most criteria necessary for its use as biomaterial. Further, eADF4(C16) particles have been shown to be well-suited for drug delivery. Here, a new method was established for particle production to reduce particle size and size distribution. Importantly, cellular uptake of these particles was shown to be poor in HeLa cells. Therefore, variants of eADF4(C16) with inversed net charge or incorporated cell penetrating peptides and receptor interacting motifs were tested, showing much better cellular uptake. Interestingly, uptake of all silk variant particles was mainly achieved by clathrin-mediated endocytosis.

Graphical abstract: Enhanced cellular uptake of engineered spider silk particles

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Supplementary files

Article information


Submitted
20 Nov 2014
Accepted
30 Dec 2014
First published
13 Jan 2015

This article is Open Access

Biomater. Sci., 2015,3, 543-551
Article type
Paper
Author version available

Enhanced cellular uptake of engineered spider silk particles

M. B. Elsner, H. M. Herold, S. Müller-Herrmann, H. Bargel and T. Scheibel, Biomater. Sci., 2015, 3, 543
DOI: 10.1039/C4BM00401A

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