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Issue 5, 2014
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Characterization of a multilayer heparin coating for biomolecule presentation to human mesenchymal stem cell spheroids

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Abstract

Mesenchymal stem cell (MSC) therapies have the potential to treat many pathologies, however, controlling cell fate after implantation remains challenging. We have used a multilayer technology to graft a range of 5 μg mL−1–5 mg mL−1 heparin onto the surface of MSC aggregates. Heparin coating does not affect cell viability (seen through LIVE/DEAD staining) and cell anti-inflammatory properties (seen through co-culture with activated monocytes) and facilitates sequestration by coated cells of a growth factor (TGF-β1) that remains bioactive. This system can maximize the therapeutic potential of MSC-based treatments because the cell surface-loaded protein could both signal the cells to influence transplanted cell fate and be released into the surrounding environment to help repair injured tissue.

Graphical abstract: Characterization of a multilayer heparin coating for biomolecule presentation to human mesenchymal stem cell spheroids

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Publication details

The article was received on 05 Nov 2013, accepted on 11 Mar 2014 and first published on 19 Mar 2014


Article type: Communication
DOI: 10.1039/C3BM60271K
Biomater. Sci., 2014,2, 666-673

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    Characterization of a multilayer heparin coating for biomolecule presentation to human mesenchymal stem cell spheroids

    J. Lei, L. T. McLane, J. E. Curtis and J. S. Temenoff, Biomater. Sci., 2014, 2, 666
    DOI: 10.1039/C3BM60271K

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