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Issue 11, 2013
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Dendrimer-entrapped gold nanoparticles modified with folic acid for targeted gene delivery applications

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Abstract

We report a new use of dendrimer-entrapped gold nanoparticles (Au DENPs) modified with folic acid (FA) as a non-viral vector for targeted gene delivery applications. In this study, amine-terminated generation 5 poly(amidoamine) dendrimers modified with FA via covalent conjugation were used as templates to synthesize gold nanoparticles with an Au salt/dendrimer molar ratio of 25 : 1. The synthesized FA-modified Au DENPs (Au DENPs-FA) were used as a non-viral vector for the delivery of plasmid DNA (pDNA) into a model cancer cell line (HeLa cells) overexpressing high-affinity FA receptors (FAR). The DNA compaction ability of the formed Au DENPs-FA was systematically characterized using a gel retardation assay, zeta potential, and dynamic light scattering. We show that similar to the Au DENPs vector without FA, the Au DENPs-FA vector was able to compact the pDNA encoding enhanced green fluorescent protein (EGFP) at an N/P ratio of 0.5. Transfection results show that the Au DENPs-FA vector enables much higher luciferase and EGFP gene expression in HeLa cells overexpressing FAR than the Au DENPs without FA, demonstrating the role played by FA-mediated targeting for enhanced gene transfection in target cells. With a lower cytotoxicity than that of the Au DENPs without FA proven by a cell viability assay, the developed FA-modified Au DENPs may be used as a promising non-viral vector for safe and targeted gene therapy applications.

Graphical abstract: Dendrimer-entrapped gold nanoparticles modified with folic acid for targeted gene delivery applications

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Publication details

The article was received on 29 May 2013, accepted on 19 Jul 2013 and first published on 08 Aug 2013


Article type: Paper
DOI: 10.1039/C3BM60138B
Biomater. Sci., 2013,1, 1172-1180

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    Dendrimer-entrapped gold nanoparticles modified with folic acid for targeted gene delivery applications

    T. Xiao, W. Hou, X. Cao, S. Wen, M. Shen and X. Shi, Biomater. Sci., 2013, 1, 1172
    DOI: 10.1039/C3BM60138B

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