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Issue 14, 2021

Development of an enzyme-linked immunosorbent assay for the detection of mebendazole in chicken and mutton

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Abstract

Mebendazole (MBZ), a synthetic benzimidazole, is most widely used for the treatment of intestinal helminthiasis. In the present study, a hapten mimicking the MBZ structure was designed by introducing propanoic acid and coupling to carrier proteins by the active ester method to immunize New Zealand rabbits. A sensitive enzyme-linked immunosorbent assay (ELISA) was developed for the analysis of MBZ in food samples. The rabbit IgG based ELISA had a half-maximum inhibition concentration (IC50) of 7.41 ng mL−1, with a limit of detection of 0.27 ng mL−1. The ELISA showed negligible cross reactivity (<1%) with structural analogs, including hydroxy-MBZ, albendazole, oxfendazole, fenbendazole, flubendazole and oxfendazole, except the value of 90.5% for amino-MBZ. The average recoveries of MBZ spiked in chicken and mutton muscle samples by the assay ranged from 84.31% to 106.28% and agreed well with those of high-performance liquid chromatography. The above results indicated that the generated anti-MBZ IgG-based ELISA showed promise in routine screening analysis of MBZ residues in food samples.

Graphical abstract: Development of an enzyme-linked immunosorbent assay for the detection of mebendazole in chicken and mutton

Article information


Submitted
24 Jan 2021
Accepted
02 Mar 2021
First published
02 Mar 2021

Anal. Methods, 2021,13, 1740-1746
Article type
Paper

Development of an enzyme-linked immunosorbent assay for the detection of mebendazole in chicken and mutton

J. He, Y. Zheng, X. Chen, N. Li, F. Tang, N. Huo, J. Wang and S. Gu, Anal. Methods, 2021, 13, 1740 DOI: 10.1039/D1AY00133G

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