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Issue 7, 2020

A highly selective colorimetric fluorescence probe for Cu2+ in aqueous media: the synthesis, DFT investigation and its application in living cells

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Abstract

A novel highly selective colorimetric fluorescence probe, POPH, has been successfully synthesized and it shows remarkable selective and sensitive response to Cu2+ in aqueous media (HEPES buffer, pH = 7.4). The fluorescence of probe POPH immediately decreased when 1.1 equiv. of Cu2+ was added to a solution of probe POPH, accompanied by color change from wathet blue to colorless under 365 nm UV lamp illumination. The possible bonding model of probe POPH with Cu2+ was examined through HRMS, FESEM, TEM, FTIR spectroscopy and DFT calculations. Moreover, the probe POPH could be used for monitoring Cu2+ in living cells using laser scanning confocal microscopy.

Graphical abstract: A highly selective colorimetric fluorescence probe for Cu2+ in aqueous media: the synthesis, DFT investigation and its application in living cells

Supplementary files

Article information


Submitted
01 Dec 2019
Accepted
23 Jan 2020
First published
25 Jan 2020

Anal. Methods, 2020,12, 943-950
Article type
Paper

A highly selective colorimetric fluorescence probe for Cu2+ in aqueous media: the synthesis, DFT investigation and its application in living cells

D. Li, L. Gong, Z. Qiao, C. Zhu, Q. Jia, H. Kong and S. Jiao, Anal. Methods, 2020, 12, 943 DOI: 10.1039/C9AY02590A

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