Lactate monitoring in droplet microfluidics: a cautionary tale in assay miniaturisation

Chi Leng Leong; Sharon Coleman; Adrian M. Nightingale; Sammer-ul Hassan; David Voegeli; Martyn G. Boutelle; Xize Niu

doi:10.1039/C9AY02070E

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Lactate monitoring in droplet microfluidics: a cautionary tale in assay miniaturisation†

Chi Leng Leong,

^ab Sharon Coleman,^a Adrian M. Nightingale,

^a Sammer-ul Hassan,

^a David Voegeli,^c Martyn G. Boutelle

*^b and Xize Niu

*^a

Author affiliations

* Corresponding authors

^a Faculty of Engineering and Physical Sciences, University of Southampton, Southampton, UK SO17 1BJ
E-mail: x.niu@soton.ac.uk

^b Department of Bioengineering, Imperial College London, South Kensington, London, UK SW7 2AZ
E-mail: m.boutelle@imperial.ac.uk

^c Faculty of Health Sciences, University of Southampton, Southampton, UK SO17 1BJ

Abstract

We present the development of a droplet-based device for real-time continuous lactate measurement, highlighting how protocols from long-established homogeneous enzymatic assays require careful re-optimisation before their migration into miniaturised droplet-based microfluidics.

This article is part of the themed collection: Bioanalytical sensors for real world applications

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DOI: https://doi.org/10.1039/C9AY02070E
Article type: Paper
Submitted: 23 Sep 2019
Accepted: 16 Nov 2019
First published: 18 Nov 2019

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Anal. Methods, 2019,11, 6119-6123

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Lactate monitoring in droplet microfluidics: a cautionary tale in assay miniaturisation

C. L. Leong, S. Coleman, A. M. Nightingale, S. Hassan, D. Voegeli, M. G. Boutelle and X. Niu, Anal. Methods, 2019, 11, 6119 DOI: 10.1039/C9AY02070E

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Analytical Methods

Lactate monitoring in droplet microfluidics: a cautionary tale in assay miniaturisation†

Abstract

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Lactate monitoring in droplet microfluidics: a cautionary tale in assay miniaturisation

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