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Issue 44, 2019
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An Fe3O4/PEDOT:PSS nanocomposite as an advanced electroconductive material for the biosensing of the prostate-specific antigen in unprocessed human plasma samples

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Abstract

A novel sandwich-type biosensor based on a signal amplification strategy of various nanocomposites was developed to monitor the prostate-specific antigen (PSA) in real samples. The engineered immunosensor was fabricated using iron oxide magnetic nanoparticles modified with a polymer poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate) [Fe3O4/PEDOT:PSS] conductive nanocomposite that utilized the immobilization of primary antibodies (biotinylated antibody (Ab1)). In order to improve the electron transfer rate, a cysteamine-capped gold nanoparticle (AuNPs-CysA) was applied for conjugation with a secondary antibody (HRP-Ab2). The fabrication procedure was investigated by square wave voltammetry. Under optimized experimental conditions, the calibration curve showed a wide linear range (0.001–0.5 μg L−1) for the PSA biomarker with a low limit of quantification of 0.001 μg L−1. Finally, satisfactory results were obtained for the determination of PSA in untreated human plasma samples, indicating the potential of the immunoassay for applications in clinical bioanalysis.

Graphical abstract: An Fe3O4/PEDOT:PSS nanocomposite as an advanced electroconductive material for the biosensing of the prostate-specific antigen in unprocessed human plasma samples

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Article information


Submitted
13 Sep 2019
Accepted
05 Oct 2019
First published
07 Oct 2019

Anal. Methods, 2019,11, 5661-5672
Article type
Paper

An Fe3O4/PEDOT:PSS nanocomposite as an advanced electroconductive material for the biosensing of the prostate-specific antigen in unprocessed human plasma samples

M. Shaban, M. Hasanzadeh and E. Solhi, Anal. Methods, 2019, 11, 5661
DOI: 10.1039/C9AY01988J

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