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Issue 19, 2019
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Automatic screening of antioxidants based on the evaluation of kinetics of suppression of chemiluminescence in a luminol–hydrogen peroxide system using a sequential injection analysis setup with a flow-batch detection cell

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Abstract

A sequential injection analysis system with sensitive chemiluminescence detection using a flow-batch detection cell was developed for the real time monitoring of kinetic profiles of antioxidant activity of phenolic substances in a luminol–Fe(III)–hydrogen peroxide system during a 60 s stop–flow period. Selected substances including coumaric acid, caffeic acid, hesperidin, ascorbic acid, resveratrol, rutin, chlorogenic acid, quercitrin, epicatechin, phloridzin, phloretin, and Trolox as a reference standard were tested with respect to different chemiluminescence signal kinetics. The evaluation of antioxidant activity was expressed as the quenching efficacy index Q. Our method has a wide working concentration range of 4–250 μmol L−1 for Trolox antioxidant activity. The real time kinetic profiles based on the chemiluminescence quenching half-life of both quick and slow antioxidants were also determined. A sample throughput of 30 h−1 was demonstrated. The antioxidant activity of apple and black elder fruit extracts was evaluated.

Graphical abstract: Automatic screening of antioxidants based on the evaluation of kinetics of suppression of chemiluminescence in a luminol–hydrogen peroxide system using a sequential injection analysis setup with a flow-batch detection cell

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Supplementary files

Article information


Submitted
21 Jan 2019
Accepted
09 Apr 2019
First published
10 Apr 2019

Anal. Methods, 2019,11, 2531-2536
Article type
Paper

Automatic screening of antioxidants based on the evaluation of kinetics of suppression of chemiluminescence in a luminol–hydrogen peroxide system using a sequential injection analysis setup with a flow-batch detection cell

G. Chládková, K. Kunovská, P. Chocholouš, M. Polášek and H. Sklenářová, Anal. Methods, 2019, 11, 2531
DOI: 10.1039/C9AY00160C

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