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Issue 6, 2019

Laccase from Botryosphaeria rhodina MAMB-05 as a biological component in electrochemical biosensing devices

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Abstract

In this work a crude laccase preparation from Botryosphaeria rhodina MAMB-05 was employed to construct an electrochemical biosensor using nanostructured carbon black. The biosensor was evaluated by differential pulse voltammetry under optimized conditions and was utilized in the determination of epinephrine (EPN). The biosensor’s storage stability was evaluated and was found to be stable over seven consecutive days with a reduction of only 7.0% in the EPN response. Under the optimum conditions developed, the biosensor provided a linear current response for the concentration of EPN in the range from 4.98 to 285 μM. The biosensor was successfully applied for determining EPN in pharmaceutical-grade EPN injection samples, and in synthetic cerebrospinal fluid, demonstrating its promising analytical performance in drug analysis.

Graphical abstract: Laccase from Botryosphaeria rhodina MAMB-05 as a biological component in electrochemical biosensing devices

Article information


Submitted
24 Dec 2018
Accepted
05 Jan 2019
First published
07 Jan 2019

Anal. Methods, 2019,11, 717-720
Article type
Communication

Laccase from Botryosphaeria rhodina MAMB-05 as a biological component in electrochemical biosensing devices

J. T. Moraes, C. A. R. Salamanca-Neto, Ĺubomír Švorc, J. G. Schirmann, A. M. Barbosa-Dekker, R. F. H. Dekker and E. R. Sartori, Anal. Methods, 2019, 11, 717 DOI: 10.1039/C8AY02805B

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