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Issue 11, 2019
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Accurate detection of matrix metalloproteinase-2 activity in clinical gastric cancer tissues using a fluorescent probe

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Abstract

Matrix metalloproteinase-2 (MMP-2) plays an important role in tumorigenesis and metastasis of gastric cancer. Therefore, specific and sensitive detection of MMP-2 is of great significance for clinical diagnosis and therapeutic evaluation of gastric cancer. Herein, we developed a new approach for accurate detection of MMP-2 activity both in gastric cancer cells and clinical tissues using a fluorescent probe Dab-PLGVRGY-FITC. The capability of the probe for detecting MMP-2 was systematically evaluated in gastric cancer MGC-803 cells and clinical gastric cancer tissues. Around 40-fold fluorescence enhancement at a wavelength of 520 nm was determined for the assays of the probe with recombinant MMP-2. The detection limit of MMP-2 was as low as 42 ng mL−1. Strong fluorescence signals were detected in MGC-803 cells and mouse gastric cancer tissues. Fluorescence signal enhancements of 11 clinical gastric cancer tissue lysates were 5.8 ∼ 9 times (7.53 ± 0.77 on average) higher than those from gastritis patients. This probe could be potentially used for early diagnosis of gastric cancer in clinics.

Graphical abstract: Accurate detection of matrix metalloproteinase-2 activity in clinical gastric cancer tissues using a fluorescent probe

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Publication details

The article was received on 22 Dec 2018, accepted on 11 Feb 2019 and first published on 15 Feb 2019


Article type: Paper
DOI: 10.1039/C8AY02789G
Anal. Methods, 2019,11, 1516-1521

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    Accurate detection of matrix metalloproteinase-2 activity in clinical gastric cancer tissues using a fluorescent probe

    F. Luan, Z. Yu, L. Yin, X. Leng, Y. Shi, J. Wang, H. Shi and W. Chen, Anal. Methods, 2019, 11, 1516
    DOI: 10.1039/C8AY02789G

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