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Issue 2, 2019
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Electrochemical determination of PIK3CA gene associated with breast cancer based on molybdenum disulfide nanosheet-supported poly(indole-6-carboxylic acid)

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Abstract

In our study, we report the development of molybdenum disulfide (MoS2) nanosheet-supported poly(indole-6-carboxylic acid) (PIn6COOH) and its usage for the electrochemical determination of circulating tumor DNA (ctDNA) such as PIK3CA gene associated with breast cancer. The MoS2 nanosheets prepared by the solvent exfoliation method from commercial bulk MoS2 were first immobilized on the surface of a carbon paste electrode; then, PIn6COOH, a novel electroactive material, was electropolymerized on the MoS2 substrate by the potentiostatic method to form a unique nanocomposite structure. The physical adsorption between aromatic In6COOH monomers and MoS2 efficiently improved the electropolymerization efficiency, resulting in increased electrochemical response of PIn6COOH. Owing to the presence of abundant carboxyl groups, probe ssDNA was covalently immobilized on the carboxyl-terminated PIn6COOH/MoS2 nanocomposite through free amines of DNA sequences based on 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide and N-hydrosulfosuccinimide crosslinking reaction. The covalently immobilized probe ssDNA could selectively hybridize with its target DNA to form dsDNA on the surface of the PIn6COOH/MoS2 nanocomposite. The self-signal changes of PIn6COOH induced by DNA immobilization and hybridization could be sensitively recognized via cyclic voltammetry and electrochemical impedance spectroscopy. This developed biosensor showed high performance of DNA hybridization with a detection limit of 1.5 × 10−17 mol L−1 for the detection of PIK3CA gene. The easy construction of this biosensor besides its fine sensitivity and very low time consumption can make this PIn6COOH/MoS2 nanocomposite very highly competitive for further applications in carcinoma diagnostic and therapeutic fields.

Graphical abstract: Electrochemical determination of PIK3CA gene associated with breast cancer based on molybdenum disulfide nanosheet-supported poly(indole-6-carboxylic acid)

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Publication details

The article was received on 07 Nov 2018, accepted on 23 Nov 2018 and first published on 27 Nov 2018


Article type: Paper
DOI: 10.1039/C8AY02425A
Anal. Methods, 2019,11, 157-162

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    Electrochemical determination of PIK3CA gene associated with breast cancer based on molybdenum disulfide nanosheet-supported poly(indole-6-carboxylic acid)

    J. Yang, X. Yin and W. Zhang, Anal. Methods, 2019, 11, 157
    DOI: 10.1039/C8AY02425A

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