Issue 3, 2019

Whole-cell biosensing by siderophore-based molecular recognition and localized surface plasmon resonance

Abstract

A siderophore-based active bacterial pull-down strategy was integrated in a localized surface plasmon resonance (LSPR) sensing platform and subsequently tested by detecting whole-cell Acinetobacter baumannii. The LSPR-based whole-cell sensing approach was previously demonstrated with aptamer-based molecular recognition motifs, and here it is extended to the powerful siderophore system, which exploits the natural bacterial need to sequester Fe(III). Specifically, a biscatecholate–monohydroxamate mixed ligand siderophore linked to a biotin via three polyethylene glycol repeating units was synthesized and immobilized on Au trigonal nanoprisms of an LSPR sensor. The resulting surface-confined biotinylated siderophore subsequently chelated Fe(III), forming a siderophore–Fe(III) complex which was shown to be competent to recognize A. baumannii. Target bacteria were captured and then detected by measuring wavelength shifts in the LSPR extinction spectrum. This siderophore pull-down LSPR biosensor approach is rapid (≤3 h detection) and sensitive – with a limit of detection (LOD) of 80 bacterial cells and a linear wavelength shift over the range 4 × 102 to 4 × 106 cfu mL−1. As intended by design, the siderophore-based biosensor was selective for A. baumannii over Pseudomonas aeruginosa, Escherichia coli, and Bacillus cereus, and was stable in ambient conditions for up to 2 weeks.

Graphical abstract: Whole-cell biosensing by siderophore-based molecular recognition and localized surface plasmon resonance

Supplementary files

Article information

Article type
Paper
Submitted
05 Oct 2018
Accepted
05 Dec 2018
First published
17 Dec 2018

Anal. Methods, 2019,11, 296-302

Whole-cell biosensing by siderophore-based molecular recognition and localized surface plasmon resonance

J. Hu, M. Ghosh, M. J. Miller and P. W. Bohn, Anal. Methods, 2019, 11, 296 DOI: 10.1039/C8AY02180E

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