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Issue 47, 2018
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A two-photon endoplasmic reticulum-targeting fluorescent probe for the imaging of pH in living cells and zebrafish

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Abstract

The pH changes of the endoplasmic reticulum (ER) are closely related to many diseases. Herein, we present a new pH-sensitive fluorescent probe (Na-pH) for the observation of ER acidification in living cells and ER stress in vivo. Na-pH consists of 1,8-naphthalimide as a fluorescence-on signal unit, 4-methylbenzenesulfonamide moiety for ER localization, and piperazine group as a pH-responsive site. Na-pH shows a sensitive fluorescence enhancement under acidic conditions, allowing it to monitor ER acidification. Meanwhile, Na-pH has a negligible response to common species, and possesses low cytotoxicity. In biological experiments, Na-pH can be used for tracking acidification during ER stress. In addition, Na-pH was also applied for two-photon imaging of pH in living tissues and zebrafish. We expect that Na-pH has great potential as a practical tool for real-time monitoring of ER acidification, which helps to reveal the pathogenesis of ER stress-related diseases.

Graphical abstract: A two-photon endoplasmic reticulum-targeting fluorescent probe for the imaging of pH in living cells and zebrafish

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Publication details

The article was received on 08 Oct 2018, accepted on 31 Oct 2018 and first published on 02 Nov 2018


Article type: Paper
DOI: 10.1039/C8AY02199F
Citation: Anal. Methods, 2018,10, 5702-5706

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    A two-photon endoplasmic reticulum-targeting fluorescent probe for the imaging of pH in living cells and zebrafish

    N. Zhang, B. Dong, X. Kong, W. Song and W. Lin, Anal. Methods, 2018, 10, 5702
    DOI: 10.1039/C8AY02199F

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