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Issue 47, 2018
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A comparison of analytical methods for measuring concentrations of 25-hydroxy vitamin D in biological samples

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Abstract

Serum 25-hydroxyvitamin D (25(OH)D) is the principal circulating metabolite of vitamin D. Several methods for measuring serum 25(OH)D have been reported. These methods include chromatographic methods (GC-MS, HPLC, and LC-MS), competitive protein-binding assay (CPBA), radioimmunoassay (RIA) and automated methods. In addition, biosensors have more recently been employed as potential candidates for routine analysis of 25(OH)D in serum. In this review, the common methods for quantification of 25(OH)D2 and 25(OH)D3 in biological samples, including the preparation of samples, are critically compared and discussed.

Graphical abstract: A comparison of analytical methods for measuring concentrations of 25-hydroxy vitamin D in biological samples

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Publication details

The article was received on 30 Sep 2018, accepted on 13 Nov 2018 and first published on 13 Nov 2018


Article type: Critical Review
DOI: 10.1039/C8AY02146E
Citation: Anal. Methods, 2018,10, 5599-5612

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    A comparison of analytical methods for measuring concentrations of 25-hydroxy vitamin D in biological samples

    M. Rezayi, M. Ghayour-Mobarhan, S. B. Tavakoly Sany, M. Fani, A. Avan, Z. Pasdar, G. A. Ferns, E. Abouzari-Lotf and I. S. Amiri, Anal. Methods, 2018, 10, 5599
    DOI: 10.1039/C8AY02146E

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