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Issue 37, 2018
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Electrochemical detection of 7-methylguanosine and DNA-methylation

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Abstract

DNA damage has been associated with cancer. Alkylating agents interact with DNA to yield a variety of products, such as 7-methylguanosine (7-mGuo), a predominant adduct, 5-methylcytosine, 3-methyladenosine and o-6-methylguanosine. This work revealed, for the first time, the oxidation mechanism of 7-mGuo on a glassy carbon electrode (GCE) using cyclic and differential pulse voltammetry, as well as impedance spectroscopy. 7-mGuo is irreversibly oxidized in a pH dependent single step reaction, predominantly under diffusion control. The anodic behaviour of 7-mGuo was compared with the anodic behaviour of other guanine derivatives. An electroanalytical method was proposed for the determination of 7-mGuo using a GCE by differential pulse voltammetry at physiological pH, with a limit of detection of 3.26 μmol L−1 and a limit of quantification of 10.88 μmol L−1. The electrochemical detection of 7-mGuo was also investigated in the presence of the potential interferents guanine, guanosine and 7-methylguanine (7-mGua). The study demonstrated that the anodic peak of 7-mGuo did not suffer from any interference from these species, since its oxidation happens at a very distinct potential. A differential pulse voltammetry study of 7-mGua and 7-mGuo in the presence of double-helix DNA (dsDNA) or DNA bases was carried out aiming to investigate the possibility of using a DNA-electrochemical biosensor for detection of DNA methylation. The electrochemical data revealed that the DNA methylation damage can be detected by a distinct signal and quantified using a DNA biosensor.

Graphical abstract: Electrochemical detection of 7-methylguanosine and DNA-methylation

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Publication details

The article was received on 26 Jul 2018, accepted on 02 Sep 2018 and first published on 03 Sep 2018


Article type: Paper
DOI: 10.1039/C8AY01667D
Citation: Anal. Methods, 2018,10, 4525-4534

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    Electrochemical detection of 7-methylguanosine and DNA-methylation

    R. F. do Nascimento, J. A. M. Nascimento, J. G. S. Montenegro, V. B. Nascimento and S. C. B. Oliveira, Anal. Methods, 2018, 10, 4525
    DOI: 10.1039/C8AY01667D

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