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Issue 37, 2018
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Simple spectrofluorimetric methods for determination of two pharmaceutically active compounds; salmeterol and trimebutine in their raw materials and pharmaceutical dosage forms in nano concentrations and testing of content uniformity

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Abstract

Novel, sensitive, and cost-effective spectrofluorimetric methods with fast procedures were used for the determination of salmeterol and trimebutine in their pharmaceutical dosage forms. The first method was based on measuring the native fluorescence of salmeterol in an aqueous solution at 415 nm after excitation at 245 nm. The second method was established using sodium dodecyl sulphate, SDS, as an organized medium to enhance the fluorescence of trimebutine. The enhanced fluorescence of trimebutine was traced at λem of 363 nm and λex of 265 nm. The calibration curves were constructed by plotting intensity of fluorescence versus concentration over the range of 6.0–60.0 and 7.0–70.0 ng mL−1 for salmeterol and trimebutine, respectively. The methods were perfectly implemented for the analysis of salmeterol and trimebutine in their pharmaceutical dosage forms. Furthermore, testing of the uniformity of salmeterol aerosol content was properly achieved. Moreover, validation parameters were applied according to ICH guidelines.

Graphical abstract: Simple spectrofluorimetric methods for determination of two pharmaceutically active compounds; salmeterol and trimebutine in their raw materials and pharmaceutical dosage forms in nano concentrations and testing of content uniformity

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Publication details

The article was received on 27 May 2018, accepted on 28 Aug 2018 and first published on 13 Sep 2018


Article type: Paper
DOI: 10.1039/C8AY01201F
Citation: Anal. Methods, 2018,10, 4511-4517

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    Simple spectrofluorimetric methods for determination of two pharmaceutically active compounds; salmeterol and trimebutine in their raw materials and pharmaceutical dosage forms in nano concentrations and testing of content uniformity

    M. I. Walash, S. Abo El Abass and M. E. Fathy, Anal. Methods, 2018, 10, 4511
    DOI: 10.1039/C8AY01201F

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