Determination of active ingredients in Chinese medicine Danning Tablets using dispersion solid-phase extraction by molecular imprinting nanomaterials coupled with HPLC-DAD

Abstract

A reliable method of magnetic molecularly imprinted polymers/dispersive solid-phase extraction (MMIPs-DSPE) was established for determination of polydatin (PD) and resveratrol (RSV) in Chinese medicine Danning Tablets® coupled with HPLC-DAD. This method was effectively used for the specific recognition and selective enrichment of target compounds and removal of matrix interferences in Chinese medicine. PD-MMIPs were obtained with PD as a template, methylacrylic acid (MAA) as a functional monomer, ethylene glycol dimethacrylate (EGDMA) as a crosslinking agent and azobisisobutyronitrile (AIBN) as an initiator. Good linearity was observed in the range of 0.01–10 μg mL⁻¹, with the limit of detection of 2.5 ng mL⁻¹ and 3.5 ng mL⁻¹ for PD and RSV, respectively. The levels of PD and RSV in Danning Tablets® were found to be 769.94 μg g⁻¹ and 130.03 μg g⁻¹, and sample recoveries were between 91.79% and 102.23% with RSD < 6%.