Jump to main content
Jump to site search

Issue 40, 2016
Previous Article Next Article

A label-free electrochemical immunosensor for the detection of cancer biomarker α-fetoprotein (AFP) based on hydroxyapatite induced redox current

Author affiliations

Abstract

A sensitive and selective electrochemical immunosensor for label-free detection of cancer biomarker α-fetoprotein (AFP) was developed based on hydroxyapatite (HAP) nanoparticles induced electrochemical current. HAP nanoparticles and graphene oxide (GO) were co-immobilized onto an electrode surface to modify the electrode. The phosphate groups contained in the HAP nanoparticles can react with molybdate to form redox molybdophosphate precipitates on the electrode surface, generating electrochemical current. GO enhanced the electrochemical current intensity due to its good conductivity. The following immobilization of the anti-AFP antibody and capture of AFP resulted in the formation of an immuno-complex layer on the electrode. This immuno-complex layer prevented the reaction of hydroxyapatite nanoparticles with molybdate, leading to the decrease of the electrochemical current, and the current change is proportional to the concentration of AFP detected. A wide linear range (0.01–10 ng mL−1) and low detection limit (5 pg mL−1) towards AFP detection were obtained. The reliability of the proposed immunosensor was evaluated by the detection of AFP in human serum samples. This study demonstrated the potential of the developed immunosensor in ultrasensitive detection of AFP or other biomarkers.

Graphical abstract: A label-free electrochemical immunosensor for the detection of cancer biomarker α-fetoprotein (AFP) based on hydroxyapatite induced redox current

Back to tab navigation

Article information


Submitted
12 Sep 2016
Accepted
15 Sep 2016
First published
16 Sep 2016

Anal. Methods, 2016,8, 7319-7323
Article type
Communication

A label-free electrochemical immunosensor for the detection of cancer biomarker α-fetoprotein (AFP) based on hydroxyapatite induced redox current

J. Sun, D. Tian, Q. Guo, L. Zhang, W. Jiang and M. Yang, Anal. Methods, 2016, 8, 7319
DOI: 10.1039/C6AY02555B

Social activity

Search articles by author

Spotlight

Advertisements