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Issue 48, 2016
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Enzyme-free fluorescent biosensor for miRNA-21 detection based on MnO2 nanosheets and catalytic hairpin assembly amplification

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Abstract

MicroRNAs (miRNAs) have attracted enormous interest due to their involvement in complex regulatory functions and for being closely linked to some cancers. Thus, qualitative and quantitative detection of miRNAs has become an urgent necessity. In this study, we report an enzyme-free fluorescent biosensor for miRNA-21 detection based on MnO2 nanosheets and catalytic hairpin assembly amplification. Results showed that the detection limit for miRNA-21 was 0.33 nM (S/N = 3) and the linearity range was from 1 to 50 nM. Compared with traditional methods, such strategy is convenient, rapid, and low-cost and it can have a promising potential for miRNA detection in cancer.

Graphical abstract: Enzyme-free fluorescent biosensor for miRNA-21 detection based on MnO2 nanosheets and catalytic hairpin assembly amplification

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Publication details

The article was received on 13 Sep 2016, accepted on 08 Nov 2016 and first published on 08 Nov 2016


Article type: Paper
DOI: 10.1039/C6AY02551J
Anal. Methods, 2016,8, 8492-8497

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    Enzyme-free fluorescent biosensor for miRNA-21 detection based on MnO2 nanosheets and catalytic hairpin assembly amplification

    W. Ouyang, Z. Liu, G. Zhang, Z. Chen, L. Guo, Z. Lin, B. Qiu and G. Chen, Anal. Methods, 2016, 8, 8492
    DOI: 10.1039/C6AY02551J

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