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Issue 37, 2016
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The fabrication of a label-free electrochemical immunosensor using an aldehyde-functionalized pyridinium salt for antibody immobilization

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Abstract

This paper reported a strategy based on the use of an aldehyde-functionalized pyridinium salt for the fabrication of a label-free electrochemical immunosensor. The aldehyde-functionalized pyridinium salt was incorporated into Nafion to form a composite film modified on the surface of an Au electrode. The aldehyde-functionalized pyridinium salt was used to capture an antibody directly and improve the conductivity of the sensing interface, which ensured the convenient fabrication of a sensitive label-free electrochemical immunosensor for α-fetoprotein detection. Under optimal conditions, α-fetoprotein was detected in the range from 0.005 to 25 ng mL−1 with a detection limit of 0.003 ng mL−1 based on a signal-to-noise ratio of 3. This strategy would offer potential promise for the convenient fabrication of immunosensors and for clinical applications.

Graphical abstract: The fabrication of a label-free electrochemical immunosensor using an aldehyde-functionalized pyridinium salt for antibody immobilization

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Publication details

The article was received on 17 Jun 2016, accepted on 04 Aug 2016 and first published on 02 Sep 2016


Article type: Paper
DOI: 10.1039/C6AY01736C
Anal. Methods, 2016,8, 6782-6786

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    The fabrication of a label-free electrochemical immunosensor using an aldehyde-functionalized pyridinium salt for antibody immobilization

    C. Zhang, Y. Pi, Y. Shen, G. Shen, Y. Zhang and S. Yao, Anal. Methods, 2016, 8, 6782
    DOI: 10.1039/C6AY01736C

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