Determination of glutathione based on NiPd nanoparticles mediated with acetaminophen

Abstract

A new method for determination of glutathione (GSH) was carried out with acetaminophen (AAP) mediation on a NiPd nanoparticle modified electrode. The as-prepared sensor exhibited excellent electrocatalytic activity for determining GSH with a good linear correlation with GSH concentration in a broad range from 0.5 to 3000 μM, a high detection sensitivity (481.69 μA mM⁻¹ cm⁻²), and a low detection limit of 0.5 μM. The mechanism for GSH oxidation was also investigated by cyclic voltammetry and linear sweep voltammetry. In addition, the proposed sensor was also successfully employed to detect GSH in real samples.