A novel biosensor nanomaterial for the ultraselective and ultrasensitive electrochemical diagnosis of the breast cancer-related BRCA1 gene

Abstract

Supramolecular ionic liquids grafted on nitrogen-doped graphene aerogels (SIL-g-(N)GAs) were used as a novel electrochemical DNA biosensor for the direct detection of the breast cancer-related BRCA1 gene. The sensor performance was monitored by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). This detection was done without any kind of enzyme, label, or sophisticated equipment. The sensor was found to be stable, reproducible, ultraselective and ultrasensitive as a limit of detection (LOD) of 2 pM was obtained by fitting the experimental data with linear equations. According to the results, this biosensor was able to perform DNA hybridization and DNA sequencing simultaneously.