Issue 13, 2016

3-Naphthyl-1-phenyl-5-(4-carboxyphenyl)-2-pyrazoline – a pyrazoline based heterocyclic dye as a fluorescent label for biomolecules containing an amino group and its evaluation using HPLC

Abstract

The use of 3-naphthyl-1-phenyl-5-(4-carboxyphenyl)-2-pyrazoline as a pre-column derivatization agent for the HPLC analysis of biomolecules containing an –NH2 group is proposed in this article. The derivatization conditions to obtain quantitative reaction were optimized by considering different parameters (time, temperature, pH and reagent concentration) using seven amino acids as model analytes. The resulting derivatives were well separated on a reversed-phase column with an isocratic elution profile and detected fluorimetrically at excitation and emission wavelengths of 370 and 480 nm, respectively. The proposed method was validated for accuracy, precision, sample stability, linearity, and detection and quantification limits and were all found to be satisfactory. Through this study, we are forecasting the emergence of pyrazoline based heterocyclic dyes as organic fluorescent markers for the labelling of diagnostically important compounds, for their determination and quantification of practical interest.

Graphical abstract: 3-Naphthyl-1-phenyl-5-(4-carboxyphenyl)-2-pyrazoline – a pyrazoline based heterocyclic dye as a fluorescent label for biomolecules containing an amino group and its evaluation using HPLC

Supplementary files

Article information

Article type
Paper
Submitted
09 Nov 2015
Accepted
18 Feb 2016
First published
19 Feb 2016

Anal. Methods, 2016,8, 2729-2736

Author version available

3-Naphthyl-1-phenyl-5-(4-carboxyphenyl)-2-pyrazoline – a pyrazoline based heterocyclic dye as a fluorescent label for biomolecules containing an amino group and its evaluation using HPLC

B. Varghese, S. N. Al-Busafi, F. O. Suliman and S. M. Z. Al-Kindy, Anal. Methods, 2016, 8, 2729 DOI: 10.1039/C5AY02952J

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