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Issue 11, 2016
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A novel colorimetric/fluorescence dual-channel sensor based on NBD for the rapid and highly sensitive detection of cysteine and homocysteine in living cells

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Abstract

A novel and simple turn-on fluorescent probe bearing 7-nitrobenz-2-oxa-1,3-diazole and benzaldehyde fragments for the rapid optical sensing of cysteine and homocysteine has been designed and synthesized. The probe has almost no background fluorescence (Φf < 0.0001) in aqueous solutions; however, it displayed fluorescence turn-on response to cysteine and homocysteine and exhibited a large bathochromic-shift (106 nm) in absorption wavelength accompanied by color changes which could be distinguished by the naked-eye. There is a good linearity with cysteine in the range of 0 to 1.37 × 10−4 M, with a detection limit of 98 nM (S/N = 3). In addition, the optical responses of the probe were investigated by density function theory (DFT) calculations. Moreover, it can be used to detect biological thiols in living cells.

Graphical abstract: A novel colorimetric/fluorescence dual-channel sensor based on NBD for the rapid and highly sensitive detection of cysteine and homocysteine in living cells

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Supplementary files

Article information


Submitted
02 Nov 2015
Accepted
12 Feb 2016
First published
16 Feb 2016

Anal. Methods, 2016,8, 2420-2426
Article type
Paper

A novel colorimetric/fluorescence dual-channel sensor based on NBD for the rapid and highly sensitive detection of cysteine and homocysteine in living cells

Y. Shen, X. Zhang, Y. Zhang, C. Zhang, J. Jin, H. Li and S. Yao, Anal. Methods, 2016, 8, 2420
DOI: 10.1039/C5AY02884A

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