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Issue 1, 2016
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Voltammetric behavior of HeLa cells on a carbon nanotube/ionic liquid modified electrode for cytotoxicity evaluation of chlorophenols

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Abstract

In the present study, the electrochemical behavior of human cervical carcinoma (HeLa) cells on a purified-multiwall carbon nanotube (p-MWCNT) and ionic liquid (IL) modified glass carbon electrode was studied. Two voltammetric signals of HeLa cells were detected, which were attributed to intracellular xanthine, guanine, hypoxanthine and adenine. The existence of these purines was further verified by the high-performance liquid chromatography (HPLC) assay. Based on the variation of the intensity of the two voltammetric signals, the cytotoxicity of 2,4-dichlorophenol (2,4-DCP), 2,4,6-trichlorophenol (2,4,6-TCP) and pentachlorophenol (PCP) was evaluated, which was also compared with the traditional methyl tetrazolium (MTT) assay. This work is expected to provide a label-free method providing more exact and perfect information to study the purine metabolism, as well as to evaluate the influence of chemicals from different perspectives.

Graphical abstract: Voltammetric behavior of HeLa cells on a carbon nanotube/ionic liquid modified electrode for cytotoxicity evaluation of chlorophenols

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Article information


Submitted
13 Oct 2015
Accepted
10 Nov 2015
First published
16 Nov 2015

Anal. Methods, 2016,8, 171-176
Article type
Paper
Author version available

Voltammetric behavior of HeLa cells on a carbon nanotube/ionic liquid modified electrode for cytotoxicity evaluation of chlorophenols

X. Zhu, H. Qin, G. Wu, J. Li, X. Yuan and D. Wu, Anal. Methods, 2016, 8, 171
DOI: 10.1039/C5AY02722E

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