A novel chemiluminescence enhanced method for determination of putrescine in shrimp based on the luminol–[Ag(HIO6)2]5− reaction

Abstract

A novel chemiluminescence (CL) method to determine putrescine was proposed based on the enhanced effect of putrescine on CL emission of the [Ag(HIO₆)₂]⁵⁻–luminol system. The enhanced degree of CL emission was proportional to the putrescine concentration. Under optimal conditions, there was a linear relationship between the increase in CL intensity and the putrescine concentration over a range of 4.0 × 10⁻⁸ to 4 × 10⁻⁶ g mL with a detection limit (3σ) of 1.76 × 10⁻⁸ g mL⁻¹. The relative standard deviation (RSD) was 3.2% for 11 replicate determinations of 1.0 × 10⁻⁶ g mL⁻¹ putrescine. The proposed CL reaction systems had potential capability for residue analysis of studied analytes in food samples. A possible mechanism of CL emission and enhanced effect was also suggested.