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Issue 20, 2015
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Simultaneous quantitation of nine kinds of (d)- and (l)-amino acid enantiomers by HPLC-MS/MS: application to the quality control of amino acid tablets

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Abstract

A simple and sensitive HPLC-MS/MS method was developed for the simultaneous determination of nine kinds of (D)- and (L)-amino acid enantiomers in amino acid tablets. 7-Chloro-4-nitrobenzoxadiazole (NBD-Cl) was selected as the derivatization reagent, and the derived amino acid enantiomers were enantioseparated on a Sumichiral OA-2500S (250 mm × 4.6 mm, 5 μm) column, using a mobile phase composed of acetonitrile–methanol (50 : 50, v/v) containing 0.5% formic acid at the flow rate of 1.0 mL min−1 with a split ratio of 1 : 3. Sensitive detection was performed with a 4000 Qtrap MS/MS system with an electrospray-ionization source in negative mode. The calibration curves for the determination of all the nine pairs of amino acid enantiomers showed good linearity (R2 > 0.999) over the investigated ranges from 0.15 to 30 μg mL−1 for the (D)-enantiomers and from 3.14 to 620 μg mL−1 for the (L)-enantiomers, respectively. The assay was reproducible with overall intra- and inter-day variations of less than 7.7%. The detection for (D)-amino acid enantiomers was selective and sensitive, and a trace amount of them could be detected and quantified, even in the presence of massive corresponding (L)-enantiomers. The validated method was successfully applied to the simultaneous quantitation of the nine kinds of (D)- and (L)-amino acid enantiomers in commercial tablets.

Graphical abstract: Simultaneous quantitation of nine kinds of (d)- and (l)-amino acid enantiomers by HPLC-MS/MS: application to the quality control of amino acid tablets

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Article information


Submitted
16 Jun 2015
Accepted
30 Aug 2015
First published
01 Sep 2015

Anal. Methods, 2015,7, 8817-8825
Article type
Paper
Author version available

Simultaneous quantitation of nine kinds of (D)- and (L)-amino acid enantiomers by HPLC-MS/MS: application to the quality control of amino acid tablets

X. Li, Y. Cui, Y. Xing, C. Lv, Q. Li and K. Bi, Anal. Methods, 2015, 7, 8817
DOI: 10.1039/C5AY01551K

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