Jump to main content
Jump to site search

Issue 16, 2015
Previous Article Next Article

Determination of 3-(4′-methylbenzylidene)camphor and its metabolite 3-(4′-carboxybenzylidene)camphor in human semen by solid-phase extraction and liquid chromatography tandem mass spectrometry

Author affiliations

Abstract

An analytical method for the determination of the controversial UV filter 3-(4′-methylbenzylidene)camphor (MBC) and its metabolite 3-(4′-carboxybenzylidene)camphor (CBC) in human semen is presented. The method is based on the simultaneous hydrolysis of phase II conjugates and protein precipitation, followed by solid-phase extraction (SPE) and liquid chromatography tandem mass spectrometry (LC-MS/MS) detection. The proposed method was validated by analyzing spiked analyte-free human semen samples. Matrix-matched calibration using an analyte-free semen pool was employed. Satisfactory recoveries (92–114%) were obtained using this calibration methodology, thus showing the accuracy of the proposed method. The limits of detection were at the low μg L−1 level (1 and 2 μg L−1 for MBC and CBC, respectively) and the intra- and inter-day precision, expressed as relative standard deviation, were in the range of 6–9% and 7–14%, respectively, depending on the analyte and the concentration level. The method was finally applied to the analysis of semen samples from two volunteers who topically applied a laboratory-made sunscreen cream containing MBC. Similar kinetic profiles performed during 72 hours were obtained for both volunteers. From this study it can be assumed that more than three days is necessary to completely metabolize or eject this UV filter.

Graphical abstract: Determination of 3-(4′-methylbenzylidene)camphor and its metabolite 3-(4′-carboxybenzylidene)camphor in human semen by solid-phase extraction and liquid chromatography tandem mass spectrometry

Back to tab navigation

Article information


Submitted
29 May 2015
Accepted
08 Jul 2015
First published
09 Jul 2015

Anal. Methods, 2015,7, 6705-6711
Article type
Paper

Determination of 3-(4′-methylbenzylidene)camphor and its metabolite 3-(4′-carboxybenzylidene)camphor in human semen by solid-phase extraction and liquid chromatography tandem mass spectrometry

I. Tarazona, A. Chisvert, Z. León and A. Salvador, Anal. Methods, 2015, 7, 6705 DOI: 10.1039/C5AY01391G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.


Social activity

Search articles by author

Spotlight

Advertisements