Issue 11, 2015

Determination of methionine enantiomers by HPLC on the cyclofructan chiral stationary phase

Abstract

The enantiomeric separation of methionine by high performance liquid chromatography on various cyclofructan type chiral stationary phases in the polar-organic separation mode was studied. The effects of the mobile phase composition and temperature on the enantioselectivity of the enantiomers were investigated. The enantioselective separation was attained on the isopropylcarbamate cyclofructan 6 chiral stationary phase with methanol/acetonitrile/acetic acid/triethylamine (75/25/0.3/0.2 v/v/v/v) as a mobile phase. The low retention and no separation were observed on the dimethylphenyl carbamate cyclofructan 7 and naphthylethyl carbamate cyclofructan 6 chiral stationary phases. Three types of detection, low-wavelength UV, polarimetric, and circular dichroism, were used. The UV detection limit for enantiomers was 11 μg mL−1 (S/N = 3) and the polarimetric and circular dichroism detection limits were 10 and 8 times lower. Good linearity was observed from 50 to 500 μg mL−1. The proposed method was applied to determine D- and L-methionine in a dietary supplement sample.

Graphical abstract: Determination of methionine enantiomers by HPLC on the cyclofructan chiral stationary phase

Article information

Article type
Paper
Submitted
27 Mar 2015
Accepted
23 Apr 2015
First published
24 Apr 2015

Anal. Methods, 2015,7, 4577-4582

Determination of methionine enantiomers by HPLC on the cyclofructan chiral stationary phase

K. Hroboňová, J. Moravčík, J. Lehotay and D. W. Armstrong, Anal. Methods, 2015, 7, 4577 DOI: 10.1039/C5AY00808E

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